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Gene silencing induced by the vector EuMY-YP∆CHS4 during the Fusarium sp. - Nicotiana benthamiana interaction
By Nidia Esther Hau Yama, Marianne Soledad Luna Rivero, Blondy Beatriz Canto Canché, Yereni Minero García, Cecilia Hernández Zepeda, Diana Sánchez Rangel, Oscar Alberto Moreno Valenzuela*
* Corresponding Author. Email: oamv@cicy.mx / Institution: Centro de Investigación Científica de Yucatán
Accepted: 10/July/2023 – Published: 31/July/2023 – DOI: https://doi.org/10.18781/R.MEX.FIT.2305-4
Abstract In the literature, RNA-based interference methods have been proposed for the control of pathogenic fungi in plants, such as virus-mediated silencing (VIGS). The objective of this work was to evaluate the functionality and efficiency of the VIGS EuMV-YP∆CHS4 vector, which contains a fragment of the Fusarium sp. chitin synthase 4 (CHS4) gene sequence, and was constructed from the EuMV-YP vector:∆AV1. The VIGS vector was evaluated under two conditions in Nicotiana benthamiana plants previously inoculated with Fusarium sp. and in plants with inoculation after the inoculation of the VIGS vector. The results showed that in both treatments with the EuMV-YP∆CHS4, a delay in leaf fall during the first days of infection and a decrease in the stem damage of almost one centimeter, compared to the control infected with the fungus. In addition, a reduction of up to 30% in the relative expression of the CHS4 gene was observed. These results suggest that the application of VIGS vectors may be efficient as a strategy to protect plants against Fusarium sp.
Keywords: Silencing RNA, Euphorbia mosaic virus-Yucatán peninsula, cross-kingdom
